Tips For Choosing Your Right ELISA Kit

The enzyme-linked immunosorbent assay (ELISA), sometimes referred to as enzyme immunoassay (EIA), is a popular, simple, and cost-effect detection technique. It uses enzymes and antibodies or antigens to identify a substance in a liquid sample, such as serum/plasma, culture supernatant, and cell/tissue lysate.

You can also buy Human PLAT/TPA ELISA Kit PicoKine from various online sources.

Here are some tips to help you choose the right ELISA kit for your sample.

1. Species studied

If the samples are from classical models such as humans, mice, and mice, it is relatively easy to find a validated ELISA kit. However, if the sample is from a non-classical model such as the monkey, a limited number of ELISA kits are available in the market. In this case, you may need to choose a species-validated kit that has homology to your sample type.

2. open analyte

You need to know exactly what type of analyte (usually protein) to find. Sandwich ELISA is usually suitable for detecting large proteins with multiple epitopes such as cytokines. Competitive ELISA is suitable for detecting small molecules such as haptens.

It is recommended that you purchase a sample-sized ELISA kit to perform a preliminary test on your sample to verify that the kit is suitable and sample sensitive.

3. Purpose of analysis

ELISA is a tool that can be used for both qualitative and quantitative analysis. Qualitative ELISA gives a simple positive or negative sample result, whereas quantitative ELISA reflects the concentration of analyte in the sample via a standard curve.

4. Type of antibody

You can contact the supplier of the ELISA kit to find out what type of antibody is used in the kit: Monoclonal or Polyclonal Antibodies In an ELISA sandwich it is sometimes useful to have a polyclonal antibody for capture and a monoclonal antibody for detection to use.